Rabbit Anti-VEGFR2抗體，VEGFR2抗體說(shuō)明書(shū)
Catalog Number :RS-0565R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background:
Hemangioblast Marker
VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a
large number of biological processes such as embryonic development, wound healing, cell proliferation, migration
and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is
autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity
or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of
Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic
tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to
enhance angiogenesis in Kaposi's sarcoma lesions.
Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus.
Cytoplasmic vesicle. Early endosome. Isoform 2: Secreted. Isoform 3: Secreted.
Tissue Specificity : Detected in cornea (at protein level). Widely expressed.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Subunit : Interacts with MYOF (By similarity). Interacts with VEGFA, VEGFC and VEGFD. Monomer in the
absence of bound VEGFA, VEGFC or VEGFD. Homodimer in the presence of bound dimeric VEGFA, VEGFC
or VEGFD. Can also form heterodimers with FLT1 and FLT4. Interacts (tyrosine phosphorylated) with FYN,
NCK1, PLCG1 and SHB. Interacts with HIV-1 Tat. Interacts with CBL. Interacts with SH2D2A/TSAD and GRB2
Specificity : Anti-VEGFR2 is a mouse monoclonal antibody
specific for VEGFR2 of human,mouse, rat，cow,dog,chk
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 12.5 kDa
Application :
– WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
lmmunohistochem1：100-500
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at
room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in
sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or
diagnostic applications.