小鼠丙二醛（MDA）酶聯(lián)免疫分析（ELISA）試劑盒
用于測(cè)定小鼠血清，血漿及相關(guān)液體樣本中丙二醛（MDA）含量。
實(shí)驗(yàn)原理：應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中小鼠丙二醛（MDA）水平。用純化的丙二醛（MDA）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入丙二醛（MDA），再與HRP標(biāo)記的MDA抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的丙二醛（MDA）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠丙二醛（MDA）濃度。
試劑盒保存：2-8℃
有效期：6個(gè)月
Mouse malondialchehyche (MDA) ELISA Kit ELISA Kit
This kit allows for the determination of MDA concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay:The kit assay Mouse MDA level in the sample，use Purified Mouse MDA antibody to coat microtiter plate wells, make solid-phase antibody, then add MDA to wells, Combined antibody which With HRP labeled goat anti-mouse become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage：2-8℃.
validity：six months.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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