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技術文章

美國Bio-rad伯樂 小型水平電泳槽Mini-Sub Cell GT Cell上海摩速

點擊次數(shù):4718 發(fā)布時間:2013-11-11

美國Bio-rad伯樂 小型水平電泳槽Mini-Sub Cell GT Cell

儀器介紹
該電泳系統(tǒng)功能zui靈活,有著zui廣泛的凝膠大小、樣品梳和分離模式可供選擇。對于普通小片段的DNA,150V 15min就可以分離開;而對于EcoRⅠ或Hind Ⅲ消化的λ噬菌體DNA產(chǎn)物60V 1.5小時也就分離開。
主要特點
1. 有帶有熒光標尺的紫外透光凝膠盤,便于隨時觀察條帶位置
2. 手輔膠選擇多樣,可以根據(jù)客戶的需要不同,制備各種尺寸的凝膠塊
3. 電泳梳產(chǎn)品選擇多樣,以方便您回收、分離等不同的需要,并滿足多通道移液器加樣
4. 電極夾更換簡單,能大大節(jié)省整個電泳過程中的耗時
技術參數(shù)
1. 凝膠盤大?。╓×L):7×7cm或7×10cm
2. 樣品通量:8-30個
3. 溴酚藍染料遷移速率:4.5cm/hr(at 75V)
4. 基座緩沖液容量: 270ml(zui多)

Separation of DNA fragments can be performed in as little as 15 minutes using the Mini-Sub Cell GT cell. EcoRI or HindIII digests of lambda DNA are easily resolved in 1.5 hours at 60 V. The Mini-Sub Cell GT cell is about one-quarter the size of the Sub-Cell GT cell and can resolve up to 30 samples. Its short, narrow format allows 7 and 10 cm runs with speed, simplicity, and economy.

The Mini-Sub Cell GT cell accommodates ReadyAgarose precast gels to save time and allow highly replicable separations, and it includes a buffer tank and safety lid with cables.

Mini-Sub Cell GT Complete System

 

UVTP Tray (cm)Combs 
Catalog #Casting GatesGel Caster7 x 7*7 x 108-Well15-WellPowerPac Basic Power Supply
170-4406   
170-4466    
170-4486  
170-4467   
164-0300  
170-4487       
164-0303      

* 7 x 7 cm tray allows casting gels in the cell using casting gates. Other gel sizes can be cast with a gel caster.

Accessories
Mini-Sub Cell GT Combs Selection Guide
Standard Combs

Catalog #WellsHeightThickness (mm)Well Width (mm)Tooth Length (mm)Well Volume (ul) in 5 mm Thick Gel
170-446415Fixed0.7531011.25
170-4465**15Fixed1.531022.5
170-433215Adjustable*1.02.610.213
170-44628Fixed0.755.51120
170-4463**8Fixed1.55.51140
170-43338Adjustable*1.05.510.227.5

*   Adjustable-height combs need comb holder, catalog #170-4331.
** Combs included in system.
Preparative Combs

Catalog #WellsHeightThickness (mm)Well Width (mm)Tooth Length (mm)Well Volume (ul) in 5 mm thick gel
170-44612 preparative
2 reference
Fixed1.520
4
10150
30
170-44601 preparative
2 reference
Fixed1.544
3
10330
22.5
170-43421 preparative)
2 reference
Adjustable*343.3
3
10.2645
45
  1. Adjustable-height combs need comb holder, catalog #170-4331.

定購信息

目錄 #描述
Mini-Sub Cell GT Systems
170-4487Mini ReadySub-Cell GT Cell, includes buffer tank, lid and electrodes, leveling bubble; accommodates 8-well and 12-well mini ReadyAgarose gels
164-0300Mini-Sub Cell GT Cell and PowerPac Basic Power Supply, 100–120/220–240 V
164-0303Mini ReadySub-Cell GT Cell and PowerPac Basic Power Supply, 100–120/220–240 V
170-4406Mini-Sub Cell GT System, with 7 x 7 cm tray
170-4466Mini-Sub Cell GT System, with 7 x 10 cm tray
170-4467Mini-Sub Cell GT System, with 7 x 10 cm tray, mini-gel caster
170-4486Mini-Sub Cell GT System, with 7 x 7 cm tray, mini-gel caster
Combs for Mini Sub-Cell GT Systems
170-433215-Well Comb, 2.6 mm wide wells, 1.0 mm thick, adjustable height
170-43338-Well Comb, 5.5 mm wide wells, 1.0 mm thick, adjustable height
170-4342Preparative Comb, 1 preparative, 2 reference wells, 3 mm thick, adjustable height
170-4460Preparative Comb, 1 preparative, 2 reference wells, 1.5 mm thick, fixed height
170-4461Preparative Comb, 2 preparative, 2 reference wells, 1.5 mm thick, fixed height
170-44628-Well Comb, 5.5 mm wide wells, 0.75 mm thick, fixed height
170-44638-Well Comb, 5.5 mm wide wells, 1.5 mm thick, fixed height
170-446415-Well Comb, 3 mm wide wells, 0.75 mm thick, fixed height
170-446515-Well Comb, 3 mm wide wells, 1.5 mm thick, fixed height
Mini-Sub Cell GT Accessories
170-4491Mini Handcasting Kit, includes 15 x 7 cm casting gates, 15-well 1.5 mm fixed-height comb, 20-well 1.5 mm fixed-height comb
170-4331Mini-Comb Holder, for Mini-Sub cell adjustable-height combs
170-4422Mini-Gel Caster
170-4432Mini-Sub Cell GT Anode (Red) Electrode Cassette
170-4433Mini-Sub Cell GT Cathode (Black) Electrode Cassette
170-4434Mini-Sub Cell GT Casting Gates, 2
170-4435Sub-Cell GT UV-Transparent Mini-Gel Tray, 7 x 10 cm (trays have 2 slots for fixed-height combs)
170-4436Sub-Cell GT UV-Transparent Mini-Gel Tray, 7 x 7 cm (trays have 2 slots for fixed-height combs)


 

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