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2023-5-17 閱讀(787)
| 文獻(xiàn)標(biāo)題 | Identifcation and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium ofcinale | ||||||||||
| 文獻(xiàn)作者 | Yong-MeiXing、Ming-MingZhao、Li-ChengGuo、Bing Li1、Juan Chen、Shun XingGuo | ||||||||||
| 期刊名稱 | Scientific Reports | 影響因子 | 4.996 | ||||||||
| 使用產(chǎn)品 | 植物鈣和鈣調(diào)素依賴型蛋白激酶家族(SnRKs CCaMK)ELISA試劑盒 | 貨號(hào) | 2Pl-KMLJ91921p | ||||||||
Identifcation and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium ofcinale
Yong-MeiXing1,4, Ming-MingZhao1,2,4, Li-ChengGuo2,3, Bing Li1, Juan Chen1?? & ShunXingGuo1??
Dendrobium ofcinale Kimura et Migo is a famous precious medicinal plant in China. Seed and seedling were c*ted with the mycorrhizal fungus Sebacina sp. CCaMK was initially cloned from D. ofcinale based on a SSH cDNA library of symbiotically germinated seeds with Sebacina sp. Phylogenetic analysis was performed among DoCCaMK and other CCaMKs. The particle bombardment technique was used to visualize DoCCaMK-GFP. qRT-PCR and western blot analysis were conducted to determine the tissue expression patterns of DoCCaMK with (SGS) and without (UGS) Sebacina sp. Furthermore, the efect of KN-93 on CCaMK expression was also examined. Using NMT the net Ca2+ fuxes and the CCaMK concentration were measured during D. ofcinale seed germination. DoCCaMK had the highest homology with Lilium longiforum CCaMK. The DoCCaMK-GFP protein localized in the nucleus and cell membrane. CCaMK expression was signifcantly upregulated after symbiosis with Sebacina sp. KN-93 could be used as an inhibitor of CCaMK to inhibit D. ofcinale seed germination. Ca2+ infux and the concentration of the CCaMK in the SGS group was signifcantly more than that of the UGS group. The characterization of CCaMK provides certain genetic evidence for the involvement of this gene during seed germination and mycorrhizal c*tion in D. ofcinale.
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